Eart

Including IUI & IVF

Five days hands-on Training course on IUI, IVF, ICSI

Course Content

Day 1

	Semen analysis
	Semen preparation
	Sperm count
	Sperm morphology and motility assessment
	Troubleshooting - clinical and lab related problems

Day 2

	Setup of an IVF laboratory
	Lab maintenance and quality management
	Controlled ovarian hyper stimulation protocols
	Follicular monitoring methods for optimal stimulation
	Ovum pickup guidelines, identification and retrieval
	Preparatory procedures for optimal oocyte handling for IVF
	Media preparation and handling
	Handling of pipettes
	Oocyte identification and handling
	Preparation of dishes for insemination
	Insemination methods for IVF
	Insemination of oocytes in culture dishes
	Troubleshooting-clinical and lab related problems

In Vitro Fertilisation

In Vitro Fertilisation

Day 3

	Different methods of sperm retrieval for ICSI
	Oocyte preparation and selection for ICSI
	Procedure for setting up of micromanipulator
	Preparation of dishes for oocyte
	Sperm immobilization and pickup
	Oocyte holding and insemination
	Culture of inseminated oocytes
	Oocyte denudation and washing
	Identification of mature oocytes
	Setting up micromanipulator
	Troubleshooting-clinical and lab related problems

Oocyte with Cumulus Cells

Oocyte with Cumulus Cells

Day 4

	Fertilization rate and success with different treatment protocols
	Embryo culture duration, decisive factors
	Embryo grading and transfer
	Assisted hatching methods
	Cryopreservation of gametes and embryos
	Troubleshooting Assisted hatching methods
	Cryopreservation of gametes and embryos
	Troubleshooting Assisted hatching methods
	Cryopreservation of gametes and embryos
	Troubleshooting

Mature Oocyte

Mature Oocyte

Day 5

	ICMR guidelines
	Discussion on gametes and embryo donation, sperm and ovum banking and surrogacy
	Prenatal Genetic Diagnosis
	Ethical aspects of assisted conception and existing laws
	Current advances in ART
	Further practice for micromanipulation and oocyte handling
	Mock cycles for perfecting timing of procedures
Reviews, feedback, multimedia education & programme completion.

Also See
Main :: One Day Orientation Course for students :: Let’s do IUI :: Let’s do Embryology :: Let’s do Calibration